Overview
The SensoLyte® 520 Enterokinase Assay Kit Fluorimetric is designed specifically for detecting enterokinase activity through a novel internally quenched FRET substrate, 5-FAM/QXL™. Enterokinase, also known as enteropeptidase, is a crucial enzyme involved in protein digestion. Produced in the duodenal wall, it converts trypsinogen to the active form trypsin, thereby activating other pancreatic digestive enzymes. Deficiencies in enterokinase can lead to serious digestive issues, impacting protein absorption and overall growth. Understanding and measuring enterokinase activity is vital for research in both gastrointestinal health and conditions like acute pancreatitis.
Key Features
- Fluorimetric detection using the internally quenched 5-FAM/QXL™ substrate
- Sensitive and specific assay for enterokinase activity
- Suitable for various research applications, including protein digestion studies
- Easy-to-follow protocol with reliable results
- Comprehensive kit including all necessary reagents
Specifications
| Product Name | SensoLyte® 520 Enterokinase Assay Kit |
|---|---|
| Application | Detection of enterokinase activity |
| Type | Fluorimetric |
| Substrate | 5-FAM/QXL™ FRET substrate |
| Storage | Store at -20°C |
FAQ
What is enterokinase and why is it important?
Enterokinase is a serine protease that activates trypsinogen to trypsin, playing a vital role in protein digestion and nutrient absorption. Its activity is crucial for proper gastrointestinal function.
How does the SensoLyte® 520 kit measure enterokinase activity?
The kit uses a fluorimetric approach, where the substrate emits fluorescence upon cleavage by enterokinase, allowing for accurate and sensitive measurement of enzyme activity.
Can this assay kit be used for clinical research?
Yes, the SensoLyte® 520 Enterokinase Assay Kit is suitable for various research applications, including clinical studies related to digestive disorders and pancreatitis.
For more products that complement your research needs, explore our Assay Kitssuch as the SensoLyte® Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit and the SensoLyte® Anti-PLP (178-191) IgG Quantitative ELISA Kit (mouse). Purchase the SensoLyte® 520 Enterokinase Assay Kit today to advance your research and ensure your experiments yield accurate, reliable results.




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