SensoLyte® 520 Aggrecanase-1 Assay Kit Fluorimetric – 1 kit
$611.00
SensoLyte® 520 Aggrecanase-1 Assay Kit Fluorimetric uses 5-FAM and TAMRA in FRET peptide substrate. Substrate used for measurement of the enzyme activity.
Overview
The SensoLyte® 520 Aggrecanase-1 Assay Kit is designed to facilitate precise measurement of aggrecanase activity, particularly targeting Aggrecanase-1 (ADAMTS-4), a crucial enzyme involved in cartilage degradation. This assay kit employs a fluorimetric approach using 5-FAM as the fluorophore and TAMRA as the quencher within a FRET peptide substrate. This setup allows for continuous monitoring of enzymatic activity, making it a valuable tool for researchers working on osteoarthritis and related conditions.
Key Features
- Quantifies Aggrecanase-1 activity with high sensitivity
- Utilizes 5-FAM and TAMRA for effective FRET detection
- Suitable for continuous measurements, enhancing data accuracy
- Ideal for studies on cartilage degradation and osteoarthritis
- Ready-to-use components streamline your workflow
Specifications
| Product Name | SensoLyte® 520 Aggrecanase-1 Assay Kit |
| Detection Method | Fluorimetric |
| Fluorophore | 5-FAM |
| Quencher | TAMRA |
| Application | Measurement of aggrecanase activity |
FAQ
What is the primary use of the SensoLyte® 520 Aggrecanase-1 Assay Kit?
This kit is primarily used to measure the activity of Aggrecanase-1, enabling researchers to study its role in cartilage degeneration, particularly in osteoarthritis.
How does the FRET mechanism work in this assay?
The assay utilizes a FRET peptide substrate where 5-FAM fluoresces when cleaved by Aggrecanase-1, and TAMRA acts as a quencher, allowing for precise monitoring of enzyme activity.
Can I use this assay kit for other aggrecanases?
This kit is specifically designed for Aggrecanase-1 (ADAMTS-4) and is not optimized for other aggrecanases. For different enzymes, consider the range of other assay kits available.
Elevate your research on cartilage health with the SensoLyte® 520 Aggrecanase-1 Assay Kit. Whether you’re studying osteoarthritis or investigating the molecular mechanisms of aggrecan degradation, this kit provides the reliability and precision you need. Order yours today and enhance your studies with our accurate and easy-to-use assay solutions!
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