Caspase 3 (Apopain) Substrate 1f, fluorogenic – 5 mg

Overview

Introducing the Caspase 3 (Apopain) Substrate 1f, fluorogenic, an essential reagent for researchers investigating apoptotic pathways. Designed specifically for the fluorimetric detection of caspase activities, this substrate allows you to quantitatively measure the activity of caspase-3 and caspase-7. By utilizing AFC-derived caspase substrates, you’ll gain greater insight into cellular processes and apoptotic signaling. With a kcat/Km of 1.3 × 10⁶ M^-1s^-1 for caspase-3, and 1.3 × 10⁴ M^-1s^-1 for caspase-7, this substrate ensures high sensitivity and specificity in your research.

Key Features

• Fluorogenic substrate for real-time detection of caspase activity
• Highly sensitive, allowing for accurate quantification
• Generates strong fluorescent signal at 500-510 nm with excitation at 370-390 nm
• Easy to use in various assay formats
• Compatible for both in vitro and in vivo studies

Specifications

FAQ

What applications can the Caspase 3 substrate be used for?

The Caspase 3 substrate is primarily used for the fluorimetric detection of caspase-3 and caspase-7 activities in various biological samples. It’s suitable for apoptosis research and other studies involving programmed cell death.

How do I use this substrate in my experiments?

To utilize the Caspase 3 substrate, dissolve the substrate in an appropriate buffer, then mix it with your sample containing the active caspases. Monitor the increase in fluorescence at the specified wavelengths to quantify enzyme activity.

Can I use this substrate for in vivo experiments?

Yes, the Caspase 3 (Apopain) Substrate 1f is designed for both in vitro and in vivo applications, making it versatile for a range of research scenarios.

Invest in the Caspase 3 (Apopain) Substrate 1f today and enhance your studies of apoptosis. This substrate is a critical tool for any lab focused on understanding cell death mechanisms. Buy now to take your research to the next level. For more options, explore our range of peptides or consider our PKG substrate for additional applications.

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