Generic 3CLpro FRET peptide substrate – 0.1 mg

Overview

The Generic 3CLpro FRET peptide substrate (0.1 mg) is designed for those engaged in SARS-CoV-2 research, particularly in studying the main protease, known as 3CLpro, nsp5, or Mpro. This enzyme plays a critical role in the viral replication process, making it a key target for therapeutic intervention. Our peptide substrate allows you to explore the enzymatic activity of 3CLpro efficiently, facilitating in-depth analyses and research outcomes.

Key Features

• Ideal for measuring the proteolytic activity of 3CLpro.
• Based on a site-specific cleavage sequence applicable to many coronavirus 3CLpro enzymes.
• Employs FRET technology: intact form has quenched fluorescence of HiLyte™ Fluor 488 by QXL® 520.
• Provides a reliable tool for enzyme kinetics studies.
• Available in a 0.1 mg size, suitable for various assays.

Specifications

FAQ

What applications can this peptide substrate be used for?

You can use the Generic 3CLpro FRET peptide substrate for various applications, including enzyme kinetics studies, drug discovery, and screening SARS-CoV-2 protease inhibitors.

How does the FRET mechanism work in this substrate?

The FRET mechanism relies on the quenching of fluorescence from the HiLyte™ Fluor 488 dye by the QXL® 520 quencher in its intact form. When the substrate is cleaved by the 3CLpro enzyme, fluorescence is restored, allowing you to measure enzyme activity accurately.

Can I use this substrate with other coronavirus research?

Yes, this peptide substrate is designed based on sequences relevant to multiple coronavirus 3CLpro enzymes, making it applicable in broader coronavirus research contexts.

Explore our full range of peptide offerings, including other enzyme substrates like PKG Substrate and Autocamtide-2or check out our specific labeled peptides like CREBtide for different applications. Start enhancing your SARS-CoV-2 research with our Generic 3CLpro FRET peptide substrate today!

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