Overview
The SensoLyte® ADHP Hydrogen Peroxide Assay Kit Fluorimetric is designed for accurate quantification of hydrogen peroxide in a variety of samples, including solutions, cell extracts, and live cells. Hydrogen peroxide plays a crucial role in numerous biological processes, particularly in free radical-induced biochemical reactions. This kit employs highly purified ADHP to provide reliable and precise measurements, making it an essential tool for researchers engaged in oxidative stress studies and enzymatic activity evaluations.
Key Features
- Quantifies hydrogen peroxide in diverse samples.
- Uses highly purified ADHP for accuracy.
- Detects oxidase activities through enzyme-coupled reactions.
- Fluorescent detection with emission at 590 nm.
- Includes calibration standards and reaction buffers.
Specifications
| Product Name | SensoLyte® ADHP Hydrogen Peroxide Assay Kit Fluorimetric |
| Detection Method | Fluorescent |
| Excitation/Emission | 573/590 nm |
| Components Included | ADHP phosphatase substrate, calibration standard, reaction buffer, stop buffer |
FAQ
What type of samples can I analyze with this kit?
The SensoLyte® ADHP kit is versatile and can be used with solutions, cell extracts, and even live cells. This flexibility makes it suitable for various research applications related to oxidative stress.
How does the assay detect hydrogen peroxide?
The kit utilizes a fluorescent detection method where the ADHP substrate is oxidized, producing a measurable signal. Specifically, it emits fluorescence at 590 nm upon oxidation, allowing for sensitive detection of hydrogen peroxide levels.
Can I use this kit to measure oxidase activity?
Yes, the SensoLyte® ADHP kit can detect oxidase activities through enzyme-coupled reactions. This makes it a valuable tool for studying enzyme kinetics and related biochemical processes.
For additional assay options, consider exploring our other assay kits, such as the SensoLyte® Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit and the SensoLyte® Anti-PLP (178-191) IgG Quantitative ELISA Kit. Each kit is designed to support your research needs effectively.
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