SensoLyte® 520 Neprilysin Activity Assay Kit Fluorimetric – 1 kit
$669.00
SensoLyte® 520 Neprilysin Activity Assay Kit Fluorimetric employs internally quenched 5-FAM/QXL™ FRET substrate for detection of neprilysin activity.
Overview
The SensoLyte® 520 Neprilysin Activity Assay Kit Fluorimetric is specifically designed for the accurate measurement of neprilysin activity in various experimental settings. This assay kit utilizes a unique internally quenched 5-FAM/QXL™ FRET substrate, providing a highly sensitive method to detect and quantify neprilysin activity. Neprilysin, known as neutral endopeptidase, plays a crucial role in peptide metabolism and is particularly significant in degrading amyloid beta-peptides in the brain. Understanding and measuring its activity is vital for research related to neurodegenerative diseases and other physiological processes.
Key Features
- Uses 5-FAM/QXL™ FRET substrate for sensitive detection
- Simple and efficient assay procedure
- Quantifies neprilysin activity in various biological samples
- Ideal for neurobiology and peptide metabolism research
- Comes with detailed protocol and support from AnaSpec
Specifications
| Item | SensoLyte® 520 Neprilysin Activity Assay Kit Fluorimetric |
| Format | Fluorimetric |
| Storage | -20°C |
| Kit Contains | 5-FAM/QXL™ FRET substrate, buffers, and complete documentation |
FAQ
What is neprilysin and why is it important?
Neprilysin is a key enzyme involved in the degradation of peptides, particularly in the brain. It helps regulate levels of neuropeptides and is implicated in various neurodegenerative conditions, making it a target for therapeutic strategies.
How do I perform the assay using the SensoLyte® 520 kit?
The assay procedure is straightforward. Simply follow the detailed protocol included with the kit. It involves preparing your samples and adding the FRET substrate, followed by measurements using a fluorescence plate reader.
Can I use this kit for samples other than brain tissue?
Yes, this assay kit is suitable for measuring neprilysin activity in a variety of biological samples such as serum, plasma, and cell cultures. Ensure proper sample preparation for accurate results.
Explore more of our assay kits to support your research needs, including the SensoLyte® Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit and the SensoLyte® Anti-PLP (178-191) IgG Quantitative ELISA Kit. The SensoLyte® 520 Neprilysin Activity Assay Kit is an essential tool for your research into neurobiology and peptide metabolism, providing reliable data that can lead to important discoveries.
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