Overview
The SensoLyte® Green Protease Assay Kit Fluorimetric is an essential tool for researchers looking to monitor generic protease activities with precision. Utilizing a casein derivative heavily labeled with a rhodamine derivative, this kit facilitates a straightforward and effective measurement of protease activity through fluorescence. The clever design ensures almost total quenching of fluorescence in the conjugate until proteases hydrolyze the casein, releasing brightly fluorescent dye-labeled peptides. With fluorescence intensity directly proportional to protease activity (Ex/Em=488/520 nm), you can confidently analyze your samples and obtain accurate results.
Key Features
- Fluorimetric detection for accurate measurement of protease activities
- Utilizes a casein derivative labeled with rhodamine for enhanced specificity
- Fluorescence intensity increase directly correlates with protease activity
- Simple and efficient assay protocol suitable for a variety of samples
- Ideal for both academic and industrial research applications
Specifications
| Item | Description |
|---|---|
| Kit Type | SensoLyte® Green Protease Assay Kit Fluorimetric |
| Applications | Protease activity detection |
| Fluorescence Excitation/Emission | 488/520 nm |
| Format | 1 kit |
FAQ
What is the main application of the SensoLyte® Green Protease Assay Kit?
This kit is primarily designed for the detection of generic protease activities by measuring changes in fluorescence intensity upon protease-mediated hydrolysis of the casein derivative.
How can I prepare the assay?
Preparation involves mixing the assay reagents as detailed in the provided protocol. Ensure you follow the steps closely for optimal results in your experiments.
Is this kit suitable for high-throughput screening?
Yes, the simple assay protocol allows this kit to be easily adapted for high-throughput screening applications, making it a versatile choice for your research needs.
Explore more of our assay kits to complement your research. Whether you’re interested in the SensoLyte® Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit or the SensoLyte® Anti-PLP (178-191) IgG Quantitative ELISA Kit, we offer a range of products designed for reliable results.




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