SensoLyte® 520 HIV Protease Assay Kit Fluorimetric – 1 kit
$642.00
SensoLyte® 520 HIV Protease Assay Kit Fluorimetric. FRET substrate, HiLyte™ Fluor 488 (fluorophore) and QXL™ 520 (quencher) measurement enzyme activities.
Overview
The SensoLyte® 520 HIV Protease Assay Kit provides a reliable and accurate method for measuring HIV protease activity. This fluorimetric assay employs a FRET peptide substrate that features HiLyte™ Fluor 488 as the fluorophore and QXL™ 520 as the quencher. When the intact FRET peptide is in place, the fluorescence emitted by HiLyte™ Fluor 488 is suppressed by QXL™ 520. Upon the action of HIV protease, this peptide is cleaved, resulting in the restoration of fluorescence, which can be continuously monitored at an excitation/emission wavelength of 490 nm/520 nm. The kit is designed for easy use, making it ideal for both research and diagnostic applications.
Key Features
- Utilizes a new FRET substrate for continuous enzyme activity measurement.
- HiLyte™ Fluor 488 provides exceptional fluorescence quantum yield.
- Compatible with standard fluorescence microplate readers.
- Simple assay protocol, saving you time in the lab.
- Perfect for both academic research and clinical laboratories.
Specifications
| Component | Description |
|---|---|
| Product Name | SensoLyte® 520 HIV Protease Assay Kit |
| Assay Type | Fluorimetric |
| Fluorophore | HiLyte™ Fluor 488 |
| Quencher | QXL™ 520 |
| Recommended Detection | Excitation/Emission 490 nm/520 nm |
FAQ
What is the purpose of the SensoLyte® 520 HIV Protease Assay Kit?
This assay kit allows you to measure HIV protease activity continuously, making it a valuable tool for HIV research and diagnostic processes.
How does the FRET technology work in this assay?
The FRET technology involves a donor (fluorophore) and an acceptor (quencher). In the intact peptide, the fluorescence is quenched. Upon cleavage, the fluorescence signal is released for measurement.
Can this kit be used with different microplate readers?
Yes, the SensoLyte® 520 HIV Protease Assay Kit is compatible with most standard fluorescence microplate readers, allowing for easy integration into your existing workflows.
Ready to enhance your research on HIV protease? Get the SensoLyte® 520 HIV Protease Assay Kit today, and explore our other assay kits for comprehensive solutions in enzyme activity measurement at Assay Kits.
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