Overview
The SensoLyte® ONPG β-Galactosidase Assay Kit Colorimetric is an essential tool for researchers aiming to measure the activity of β-galactosidase, a widely recognized reporter enzyme. This kit employs o-nitrophenyl β-D-galactopyranoside (ONPG) as the chromogenic substrate, allowing for easy detection and quantification of β-galactosidase activity in your samples. Whether you’re studying gene expression, protein-protein interactions, or transfection efficiency in mammalian cells, this assay kit serves as a reliable resource in your experiments.
Key Features
- Utilizes ONPG as a substrate for accurate measurement of β-galactosidase activity.
- Ideal for applications in gene expression and protein interaction studies.
- Simple colorimetric detection method.
- Compatible with various sample types, including transfected cells and tissues.
- Comes with all necessary reagents for up to 100 assays.
Specifications
| Product Name | SensoLyte® ONPG β-Galactosidase Assay Kit Colorimetric |
|---|---|
| Format | Colorimetric |
| Substrate | ONPG |
| Reagents | Complete kit for 100 assays |
FAQ
What is β-galactosidase used for?
β-Galactosidase is commonly used as a reporter enzyme in molecular biology to study gene expression and protein interactions. Its activity can provide insights into gene regulation and transfection efficacy.
How do I perform the assay with this kit?
The kit includes detailed instructions on how to prepare your samples, add the reagents, and measure the absorbance to determine β-galactosidase activity. It’s designed to be straightforward and user-friendly, even for those new to assay techniques.
Can this assay kit be used with mammalian cells?
Yes, the SensoLyte® ONPG β-Galactosidase Assay Kit is suitable for measuring β-galactosidase activity in various mammalian cell lines and tissues, making it a versatile choice for your research needs.
For related assays, check out our SensoLyte Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit or explore our full range of Assay Kits for more options that might complement your studies.




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