SensoLyte® 520 FAP (Seprase) Assay Kit *Fluorimetric* – 100 Assays (96-well plate)

Overview

The SensoLyte® 520 FAP (Seprase) Assay Kit is your go-to solution for screening enzyme inhibitors or continuously monitoring FAP (fibroblast activation protein) activity. This fluorimetric assay kit uses a HiLyte Fluor™488/QXL® FRET substrate that is specifically cleaved by FAP but remains untouched by DPPIV. Upon cleavage, the FRET substrate splits into two parts, resulting in the release of a brilliant HiLyte Fluor™488 fluorescence, making it easy to monitor enzyme activity with precision. You can conduct this assay in a 96-well plate setup, allowing for up to 100 assays, so you can focus on your research without worrying about running out.

Key Features

• Designed for screening of enzyme inhibitors and continuous assay of FAP activity
• Uses HiLyte Fluor™488/QXL® FRET substrate for specific fluorescence detection
• Separation of substrate fragments leads to detectable fluorescence at excitation/emission of 490 nm/520 nm
• Compatible with a standard 96-well plate format for high-throughput screening
• Eliminates interference from common substances, enhancing accuracy

Specifications

FAQ

What types of samples can I use with this assay kit?

You can utilize a wide range of biological samples, including cell lysates and purified enzymes, making this kit flexible for various research applications.

How long does the assay take to complete?

The assay protocol is straightforward and can typically be completed within a few hours, allowing for efficient processing and quick results.

Can this kit be used for high-throughput screening?

Yes, the SensoLyte® 520 FAP Assay Kit is designed for high-throughput applications, especially with its 96-well plate format, making it suitable for large-scale studies.

If you’re interested in expanding your research capabilities, you might want to check out our other assay kits, such as the SensoLyte® Anti-Human MOG IgG Specific ELISA Kit and the SensoLyte® Anti-PLP IgG Quantitative ELISA Kit. These products complement the SensoLyte® 520 FAP Assay Kit and enhance your research toolbox.

Related Products

• SensoLyte® Anti-Mouse MOG (1-125) IgG Quantitative ELISA Kit Colorimetric – 1 kit

Browse more in Assay Kits.