Biotin-X NTA [Biotin-X nitrilotriacetic acid, tripotassium salt] – 5 mg

Overview

Biotin-X nitrilotriacetic acid, tripotassium salt (biotin-X NTA) is your go-to reagent for accurately detecting polyhistidine-tagged biomolecules immobilized on membranes. This versatile compound features an NTA moiety that forms stable chelates with Ni++ ions, which are essential for binding His-tagged proteins. By utilizing biotin-X NTA, you can enhance the efficiency of your assays and streamline your research outcomes.

Key Features

• Highly effective for detecting polyhistidine-containing biomolecules.
• Facilitates easy immobilization on membranes.
• Complements detection procedures involving streptavidin-conjugates, such as streptavidin-HRP.
• Can be removed from His-tagged molecules at pH 4.8, allowing for reprobing.
• Ideal for various assay applications in research and diagnostic labs.

Specifications

FAQ

How does biotin-X NTA enhance the detection of His-tagged proteins?

Biotin-X NTA effectively chelates Ni++ ions, creating a stable complex with His-tagged proteins. This complex can then be readily detected using various streptavidin conjugates.

Can biotin-X NTA be reused in experiments?

Yes, biotin-X NTA can be removed from His-tagged molecules at a pH of 4.8, allowing you to reprobe the blot without losing your sample.

Where can I find more information about streptavidin products?

You can explore our range of streptavidin products to find additional reagents that complement your research needs.

When you purchase biotin-X NTA, you’re investing in more than just a reagent; you’re investing in reliable results. Whether you’re conducting basic research or developing diagnostic assays, biotin-X NTA is an essential tool for your laboratory.

Related Products

• Streptavidin, recombinant
• Biotin (d-Biotin)

Browse more in Non-fluorescent.