6-FAM [6-Carboxyfluorescein]

Overview

The 6-FAM [6-Carboxyfluorescein] is an essential fluorescent labeling dye used primarily in the sequencing of nucleic acids and the labeling of nucleotides. As a superior alternative to FITC reagents, 6-FAM offers enhanced stability and improved conjugation yields, making it a preferred choice for researchers in various fields. This fluorescent dye is invaluable for applications requiring reliable and consistent results when working with bioconjugates.

Key Features

• Single isomer of carboxyfluorescein
• High stability and resistance to hydrolysis
• Better conjugation yields compared to FITC
• Less stringent reaction conditions required
• Ideal for nucleic acid sequencing and labeling

Specifications

FAQ

What makes 6-FAM a better choice than FITC?

6-FAM provides greater stability and results in more reliable conjugates, making it an excellent alternative, especially when hydrolysis resistance is critical.

How do I use 6-FAM for labeling nucleotides?

To label nucleotides effectively, you can react 6-FAM with amine groups present in the nucleotide structure, forming stable carboxamides that maintain their fluorescent properties during analysis.

Is 6-FAM suitable for all types of nucleic acid studies?

Yes, 6-FAM is versatile and can be used in a variety of nucleic acid studies, including PCR, sequencing, and other labeling applications. For additional options, check out our 5-FAM [5-Carboxyfluorescein].

Shopping for high-quality fluorescent labeling dyes? The 6-FAM [6-Carboxyfluorescein] is ready for your next project, ensuring accuracy and reliability in your experiments. Explore the benefits of 6-FAM and enhance your research capabilities today.

Related Products

• 6-FAM, SE [6-Carboxyfluorescein, SE]
• 5-FAM, SE [5-Carboxyfluorescein, SE]

Browse more in Green (500 -550 nm).