5-FAM [5-Carboxyfluorescein]

Overview

5-FAM, or 5-Carboxyfluorescein, is a widely used green fluorescent reagent, ideal for in situ labeling of peptides, proteins, and nucleotides. Recognized for its superior stability and ease of use, 5-FAM delivers high-quality results in various biochemical applications. Researchers often prefer this versatile dye over other fluorescent agents due to its reliable performance in bioconjugation processes.

Key Features

• High stability compared to FITC reagents, ensuring longevity in experiments
• Easy amine-reactive succinimidyl esters formulation for straightforward conjugation
• Generates carboxamides that resist hydrolysis, enhancing reliability
• Less stringent reaction conditions that contribute to better conjugation yields
• Ideal for labeling peptides, proteins, and nucleotides in various assays

Specifications

FAQ

What applications can 5-FAM be used for?

5-FAM is effective in labeling peptides, proteins, and nucleotides, making it suitable for a wide range of biochemical assays. Its stability and ease of use make it an excellent choice for researchers.

How does 5-FAM compare to other fluorescent dyes?

While many researchers use FITC reagents, they can suffer from stability issues. 5-FAM offers greater stability and better conjugation yields under less stringent conditions, making it a preferred choice in various applications.

Can I combine 5-FAM with other labeling dyes?

Yes, you can combine 5-FAM with other fluorescent dyes, such as 6-FAM or its derivatives, to create multiplex assays. This can enhance your experiment’s versatility and depth of detection.

If you’re looking for a reliable fluorescent labeling dye that excels in stability and performance, consider adding 5-FAM to your repertoire. Shop now and experience the difference it can make in your research.

Related Products

• 6-FAM [6-Carboxyfluorescein]
• 6-FAM, SE [6-Carboxyfluorescein, SE]
• 5-FAM, SE [5-Carboxyfluorescein, SE]

Browse more in Green (500 -550 nm).