Overview
Introducing the HiLyte™ Fluor 647 amine, a carbonyl-reactive fluorescent labeling dye that makes your assays more efficient and reliable. This innovative product generates conjugates that are slightly red-shifted compared to Cy®5 dyes, allowing for a better fit with filters designed to work optimally with Cy®5. Whether you’re conducting fluorescence polarization-based assays or other experimental designs, the HiLyte™ Fluor 647 amine will enhance your overall results, providing potentially superior performance.
Key Features
- Carbonyl-reactive fluorescent labeling dye
- Produces slightly red-shifted conjugates compared to Cy®5
- Optimized for use with Cy®5 filters
- Fluorescence quantum yield of 0.33
- Extinction coefficient of 250,000
- Fluorescence lifetime of 1.0 ns
Specifications
| Property | Value |
|---|---|
| Extinction Coefficient (M-1cm-1) | 250,000 |
| Fluorescence Quantum Yield | 0.33 |
| Fluorescence Lifetime (ns) | 1.0 |
| Category | Red (620 -750 nm) |
FAQ
What are the main applications for HiLyte™ Fluor 647 amine?
The HiLyte™ Fluor 647 amine is primarily used in fluorescence assays, including fluorescence polarization assays, where higher performance is needed compared to traditional Cy®5 dyes.
Can I use it with other labeling dyes?
Yes, the HiLyte™ Fluor 647 amine can be used alongside other fluorescent dyes like HiLyte™ Fluor 594 succinimidyl ester and CyLyte Fluor 5, acid to create multi-color assays, providing versatility for your experimental designs.
How can I ensure optimal performance with this dye?
For the best results, pair HiLyte™ Fluor 647 amine with suitable filters designed for Cy®5 and follow proper labeling protocols to achieve efficient conjugation.
Unlock your research potential by purchasing the HiLyte™ Fluor 647 amine from AnaSpec today. Our high-quality fluorescent labeling dye is for sale now, giving you the edge in your experimental outcomes.
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