HiLyte™ Fluor 647 acid – 5 mg

Overview

HiLyte™ Fluor 647 acid is a premium fluorescent dye designed for precise and reliable labeling applications. This dye is particularly valued in the research community for its superior performance compared to similar products on the market, such as Cy®5 dyes. Offering slightly red-shifted spectra, HiLyte™ Fluor 647 acid matches well with filters designed for Cy®5. However, its robust performance extends beyond mere compatibility; its total fluorescence outshines that of competitive secondary antibody conjugates, making it an excellent choice for your labeling needs.

Key Features

• High total fluorescence compared to Cy®5 conjugates
• Minimal change in absorption or fluorescence spectra during conjugation
• Compatible with a variety of proteins, oligonucleotides, and nucleic acids
• Designed for optimal performance in red (620 – 750 nm) applications

Specifications

FAQ

What applications can HiLyte™ Fluor 647 acid be used for?

This dye is ideal for a variety of applications, including immunofluorescence, flow cytometry, and other assays requiring reliable fluorescent labeling.

How does HiLyte™ Fluor 647 acid compare to Cy®5?

HiLyte™ Fluor 647 acid not only matches the red-shifted spectra of Cy®5 but also offers significantly higher total fluorescence, enhancing the visibility of your experiments.

Can HiLyte™ Fluor 647 acid be conjugated to nucleic acids?

Yes, this dye exhibits minimal alteration in its absorption or fluorescence spectra, making it suitable for conjugation with nucleic acids, proteins, and other biomolecules.

When you invest in HiLyte™ Fluor 647 acid, you’re choosing a dye that outperforms many alternatives in both fluorescence output and spectral stability. For more fluorescent dye options, explore our Red (620 – 750 nm) collection or check out our HiLyte™ Fluor 594 succinimidyl ester for another excellent labeling choice.

Related Products

• CyLyte Fluor 5, acid
• CyLyte Fluor 5, NHS ester