Overview
B-Phycoerythrin (B-PE) is a highly fluorescent protein sourced from cyanobacteria and eukaryotic algae, making it an ideal choice for a variety of labeling applications. With its primary absorption peak at 545 nm and a secondary peak at 563 nm, this orange fluorescent protein is extensively utilized in flow cytometry and multi-color immunofluorescent staining methods. The unique structure of B-PE, consisting of α, β, and γ subunits arranged as (αβ)6γ, contributes to its remarkable brightness and photostability, positioning it as a preferred alternative to conventional organic fluorophores. If you need reliable and vibrant fluorescence for your research, the B-PE labeled streptavidin is a strong option.
Key Features
- Strong orange fluorescence between 565-620 nm
- High photostability ideal for prolonged imaging
- Optimal for flow cytometry applications
- Compatible with primary and secondary antibodies
- Perfect for multi-color staining experiments
Specifications
| Product Name | B-PE (B-Phycoerythrin) |
| Quantity | 1 mg |
| Emission Range | 565-620 nm |
| Source | Cyanobacteria and eukaryotic algae |
FAQ
How is B-PE used in flow cytometry?
B-PE is primarily used as a fluorescent label for antibodies in flow cytometry. Its high brightness and photostability enhance signal detection, allowing for the analysis of multiple markers simultaneously.
What are the advantages of using B-PE over traditional fluorophores?
B-PE offers superior brightness and photostability compared to conventional organic fluorophores, reducing the chances of fading during extended imaging sessions. This ensures more accurate and reliable results in your experiments.
Can B-PE be used for multi-color staining?
Absolutely. B-PE is compatible with primary and secondary antibodies, making it an excellent choice for multi-color immunofluorescent staining applications where multiple targets need to be detected simultaneously.
For all your fluorescence needs, consider integrating B-phycoerythrin into your labeling protocols. Its intense orange fluorescence is unmatched for various assays, including those involving various proteins and nucleic acids. Don’t miss out on this critical reagent that can elevate your research. You can also explore our other products like the Protein A-HiLyte™ Fluor 555 Conjugate and 5-FITC cadaverine for enhanced experimental options.
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