Overview
5-IAF [5-Iodoacetamidofluorescein] is a powerful fluorescent dye specifically designed for thiol modifications in proteins. As a member of the iodoacetamide family, it is recognized for its reliability and effectiveness in biochemical research. This product is essential for researchers focusing on protein labeling and detection. By choosing 5-IAF, you can enhance your experiments with a dye that delivers exceptional fluorescence and clear results.
Key Features
- High-performance fluorescent dye for thiol modification.
- Optimal absorption and emission wavelengths in the green spectrum (500-550 nm).
- Easily incorporated into various biochemical assays.
- Compatible with both peptide and protein applications.
- Suitable for a range of labeling and detection methods.
Specifications
| Product Name | 5-IAF [5-Iodoacetamidofluorescein] |
|---|---|
| Quantity | 25 mg |
| Excitation Wavelength | 500 nm |
| Emission Wavelength | 550 nm |
FAQ
What is 5-IAF used for?
5-IAF is primarily used for thiol modification in proteins. It helps in enhancing the detection and labeling of proteins in various assays, making it a valuable tool in research.
How do I use 5-IAF in my experiments?
To use 5-IAF, mix the dye with your protein sample under appropriate conditions. Follow the specific protocol guidelines for optimal results, ensuring the dye interacts effectively with thiol groups present in your proteins.
Is 5-IAF compatible with other fluorescent dyes?
Yes, 5-IAF can be used alongside other fluorescent dyes like 6-FAM [6-Carboxyfluorescein] or 5-FAM [5-Carboxyfluorescein] for multiplexing experiments. Just make sure to account for their respective excitation and emission wavelengths during your experiments.
5-IAF is a dependable option for your fluorescent labeling needs. Its precision in thiol modifications is unmatched, allowing you to confidently pursue your research goals. Don’t miss out on the potential this dye brings to your assays; shop now and enhance your protein analysis with 5-IAF.
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