Overview
The AnaTag™ HiLyte™ Fluor 488 Microscale Protein Labeling Kit is designed for efficient protein labeling, allowing researchers to conduct up to three conjugation reactions with each kit, labeling up to 200 µg of IgG. This microscale kit is ideal for those in proteomics, immunology, and cellular imaging who need reliable and effective protein conjugation solutions.
Key Features
- Amine-reactive fluorescent labeling dye for protein conjugates.
- Compatible with filters designed for fluorescein, offering enhanced imaging.
- More photostable than traditional FITC, enabling longer observation times.
- Fluorescence detection at an emission wavelength of 523±10 nm when excited at 499±10 nm.
- Perfect for a variety of research applications, including diagnostics and therapeutics.
Specifications
| Property | Details |
|---|---|
| Labeling Dye | HiLyte™ Fluor 488 |
| Labeling Capacity | Up to 200 µg IgG |
| Excitation Wavelength | 499±10 nm |
| Emission Wavelength | 523±10 nm |
| Reactions per Kit | Three |
FAQ
What applications can I use this kit for?
The AnaTag™ HiLyte™ Fluor 488 Microscale Protein Labeling Kit is applicable in various fields, including cell biology, immunology, and biochemistry, making it versatile for many research projects.
How does the stability of HiLyte™ Fluor 488 compare to FITC?
HiLyte™ Fluor 488 is more photostable than FITC, providing researchers with a longer window to capture high-quality images, which is crucial for precise analysis.
Is there a specific protocol I should follow for labeling?
Yes, detailed protocols are included with the kit to ensure optimal results for your protein labeling experiments. Following these protocols is essential for achieving the best performance.
For more advanced needs, consider comparing it with our AnaTag™ 5 – FITC Protein Labeling Kit or the AnaTag™ 5 – FITC Microscale Protein Labeling Kit. Both offer excellent options for various labeling tasks. Explore the complete range of our green fluorescent dyes to find the perfect fit for your research.



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