Overview
HiLyte™ Fluor 405 succinimidyl ester (SE) is your go-to choice for blue fluorescent labeling, perfect for a wide range of applications in research and diagnostics. Ideal for labeling peptides, proteins, and antibodies, this fluorescent dye mirrors the capabilities of Alexa Fluor™ 405, providing excellent performance in your experiments. With an excitation/emission spectrum of 404/428 nm, HiLyte™ Fluor 405 offers reliable quantification and visualization in various biological assays.
Key Features
- Ex/Em = 404/428 nm, compatible with various laser systems
- Amine reactive for targeted labeling of biomolecules
- Extinction coefficient (M-1cm-1): 35,000
- Fluorescence quantum yield: 0.54
- Suitable for a variety of applications, including flow cytometry and microscopy
Specifications
| Product Name | HiLyte™ Fluor 405 succinimidyl ester (SE) |
| Quantity | 1 mg |
| Category | Blue (<500 nm) |
| Amine Reactive | Yes |
FAQ
What are the applications of HiLyte™ Fluor 405?
This dye is ideal for various applications including flow cytometry, microscopy, and immunohistochemistry, making it versatile for many experimental setups.
How do I use HiLyte™ Fluor 405 for labeling?
To use HiLyte™ Fluor 405, simply mix the dye with your target biomolecule in an amine-reactive environment, following specific protocols to achieve optimal labeling efficiency.
Can I use HiLyte™ Fluor 405 with other fluorescent dyes?
Yes, HiLyte™ Fluor 405 can be used in combination with other fluorescent dyes. Ensure that the excitation and emission spectra of the dyes do not overlap for optimal results.
Are you looking to enhance your research with reliable fluorescent labeling? HiLyte™ Fluor 405 is available for purchase now on our site. Explore our full range of fluorescent labeling dyes and find the perfect fit for your project today.
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