Overview
The Caspase 3 (Apopain) Substrate 1m, fluorogenic is an essential tool for researchers investigating cell apoptosis and signaling pathways. Specifically designed for the fluorimetric detection of caspase activities, this substrate allows you to monitor caspase-3 and caspase-7 activity with precision. The substrate cleaves AMC-derived peptides, resulting in a strong fluorescent signal that can be easily detected, aiding in your understanding of various biological processes.
Key Features
- High specificity for caspase-3 and caspase-7 enzymatic activity.
- Fluorogenic response with maximal fluorescence at 440-460 nm.
- AMC-derived substrate with a Km value of 9.7 µM for caspase-3.
- Efficient detection of apoptosis in cellular assays.
- Available in 5 mg quantity, ideal for numerous experiments.
Specifications
| Product Name | Caspase 3 (Apopain) Substrate 1m, fluorogenic |
|---|---|
| Quantity | 5 mg |
| Km for Caspase-3 | 9.7 µM |
| Km for Caspase-7 | 11 µM |
| Excitation Wavelength | 340-350 nm |
| Emission Wavelength | 440-460 nm |
FAQ
What applications can Caspase 3 (Apopain) Substrate be used for?
This substrate is used extensively in cellular apoptosis assays, enabling researchers to measure caspase activity accurately in various models.
How do I prepare the substrate for use?
To prepare the Caspase 3 substrate, simply dissolve the powdered substrate in an appropriate buffer solution, ensuring the final concentration matches your experimental requirements.
Where can I find related products?
You can explore additional options like PKG Substrate or Autocamtide-2 to complement your studies on protein function and enzyme kinetics.
Enhance your research with our selection of products, including peptides and labeled proteins designed for versatility and high performance.




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