Overview
The SensoLyte® 520 HCV Protease Assay Kit provides a reliable and efficient way to measure the activity of the HCV NS3/4a protease enzyme. Utilizing a special FRET peptide substrate that combines the fluorophore 5-FAM and the quencher QXL™ 520, this kit allows for continuous monitoring of enzyme activity. When the FRET peptide is cleaved by the HCV protease, the fluorescence signal becomes detectable, making it an effective tool for researchers studying HCV’s enzymatic function and its influence on viral replication.
Key Features
- Continuous measurement of enzyme activity via fluorimetric assays
- Utilizes 5-FAM as a fluorophore for enhanced signal detection
- Employs QXL™ 520 as a quencher for precise measurements
- Compatible with various fluorescence detection instruments
- Fast and easy protocol with minimal handling
Specifications
| Kit Type | Fluorimetric Assay Kit |
| Excitation/Emission | 490 nm/520 nm |
| Storage Temperature | -20°C |
| Quantity | 1 Kit |
FAQ
How does the SensoLyte® 520 HCV Protease Assay Kit work?
This assay kit employs a FRET peptide substrate that releases a fluorescence signal when cleaved by the HCV protease, enabling continuous monitoring of enzyme activity.
What instruments are compatible with this assay?
Most standard fluorescence detection systems can be used with the SensoLyte® 520 HCV Assay Kit, ensuring versatility in laboratory applications.
Can I use this kit for other proteases?
This assay is specifically optimized for HCV NS3/4a protease. For other enzymes, consider our selection of assay kits designed for different targets.
Ready to advance your research? Buy the SensoLyte® 520 HCV Protease Assay Kit today and experience the ease of enzyme activity measurement.




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