SensoLyte® 520 MMP-7 Assay Kit Fluorimetric – 1 kit
$576.00
SensoLyte® 520 MMP-7 Assay Kit Fluorimetric uses 5-FAM (fluorophore) QXL520™ (quencher) labeled FRET peptide substrates for measurement enzyme activities.
Overview
The SensoLyte® 520 MMP-7 Assay Kit Fluorimetric provides a precise and efficient way to measure the activity of MMP-7 (matrilysin, PUMP), an enzyme important in biological processes like tissue remodeling, cell proliferation, and host defenses. This assay kit employs a sophisticated design using 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET (Förster resonance energy transfer) peptide substrates. When intact, the fluorescence of 5-FAM is quenched by QXL520™, but upon the cleavage of the FRET peptide by MMP-7, the fluorescence signal is restored, allowing for continuous monitoring and quantification of enzyme activity.
Key Features
- Utilizes 5-FAM and QXL520™ for accurate fluorescence measurement
- Continuous detection of enzyme activity
- Ideal for research in tissue remodeling and physiological processes
- Easy protocol for straightforward implementation
- Single kit format for convenience and efficiency
Specifications
| Component | Details |
|---|---|
| Assay Type | Fluorimetric |
| Fluorophore | 5-FAM |
| Quencher | QXL520™ |
| Target Enzyme | MMP-7 |
| Format | 1 kit |
FAQ
What biological processes does MMP-7 influence?
MMP-7 is involved in various biological events, including tissue remodeling and immune responses, making it crucial for understanding certain diseases.
How does the fluorimetric assay work?
The assay works by measuring the fluorescence recovery after the cleavage of the substrate by MMP-7, providing a continuous readout of enzyme activity.
Can I use this kit for high-throughput screening?
Yes, the SensoLyte® 520 MMP-7 Assay Kit is designed for versatility and can be adapted for high-throughput applications within the lab.
If you’re looking to measure enzyme activities effectively, shop the SensoLyte® 520 MMP-7 Assay Kit today. Visit our Assay Kits section for more advanced solutions, like the SensoLyte® Anti-PLP (178-191) IgG Quantitative ELISA Kit (mouse) Colorimetric, or explore our extensive range of related products for your research needs.
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