SensoLyte® 520 TEV Protease Assay Kit Fluorimetric – 1 kit
$528.00
SensoLyte® 520 TEV Protease Assay Kit Fluorimetric t uses TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL™ 520 quencher.
Overview
The SensoLyte® 520 TEV Protease Assay Kit is an essential tool for researchers looking to study the Tobacco Etch Virus (TEV) protease, a critical enzyme utilized in the removal of fusion tags from recombinant proteins. This kit leverages a TEV-specific substrate, Glu-Asn-Leu-Tyr-Phe-Gln-Gly, which is labeled with the fluorescent dye 5-FAM and paired with the QXL™ 520 quencher. This unique formulation allows for straightforward monitoring of proteolytic activity through fluorescence, giving you a clean and efficient way to evaluate TEV protease functionality. Available now for immediate purchase, the SensoLyte® 520 TEV Protease Assay Kit is a must-have for any laboratory focused on protein purification and analysis.
Key Features
- Uses a TEV-specific substrate for enhanced specificity.
- 5-FAM dye provides high sensitivity and clear fluorescent detection.
- Active at low temperatures, enabling flexibility in experimental conditions.
- Simple protocol allows for easy implementation in your lab.
- Ideal for studying fusion protein activity and verification.
Specifications
| Component | Description |
|---|---|
| Assay Type | Fluorimetric |
| Substrate | 5-FAM labeled TEV specific substrate |
| Quencher | QXL™ 520 |
| Storage Temperature | 4 °C |
FAQ
What is the optimal temperature for using the SensoLyte® 520 TEV Protease Assay Kit?
The TEV protease remains active at temperatures as low as 4 °C, allowing you flexibility in your experimental setup.
Can I use this kit for other proteases?
This assay kit is specifically designed for TEV protease and may not accurately measure the activity of other proteases.
How do I interpret the results from the assay?
The increase in fluorescence signals a successful cleavage event, allowing you to quantify the activity of TEV protease in your samples.
For your research needs, consider integrating other assay kits such as the SensoLyte® Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit or the SensoLyte® Anti-PLP (178-191) IgG Quantitative ELISA Kit to enhance your experimental capabilities. Shop now and facilitate your research with our comprehensive selection of high-quality assay kits.
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