Tetramethylrhodamine-5-maleimide – 5 mg

Overview

Tetramethylrhodamine-5-maleimide is a premier fluorescent labeling dye, specifically designed for thiol modifications of peptides and proteins. This valuable reagent is a go-to choice for researchers looking to enhance their protein labeling capabilities. By targeting cysteine residues, which may naturally occur in proteins or arise from the reduction of cystines, this compound allows for precise labeling without the common pitfalls associated with other reagents. Unlike iodoacetamides, maleimides like Tetramethylrhodamine-5-maleimide do not interact with histidines or methionines in physiological environments, making it a safer and more reliable option for your labeling processes.

Key Features

• Ideal for thiol modifications of peptides and proteins
• Targets cysteine residues specifically
• Non-reactive towards histidines and methionines under physiological conditions
• Fluorescent dye with emission in the orange spectrum (565-620 nm)
• 5 mg quantity suitable for various applications

Specifications

FAQ

What types of applications is Tetramethylrhodamine-5-maleimide suitable for?

This product is primarily used for the labeling of peptides and proteins where thiol modifications are required. Its specific targeting of cysteine residues makes it especially useful in bioconjugation and fluorescence studies.

How does Tetramethylrhodamine-5-maleimide compare to other labeling dyes?

Compared to other labeling dyes like 5(6)-TAMRA, Tetramethylrhodamine-5-maleimide offers advantages in specificity for thiol groups during protein modifications while maintaining a strong fluorescent signal.

Can this dye be used in live cell imaging?

While Tetramethylrhodamine-5-maleimide is a reliable choice for many applications, its suitability for live cell imaging will depend on the specific experimental conditions. Always conduct preliminary tests to ensure compatibility with your system.

If you’re looking for effective fluorescent labeling, consider adding Tetramethylrhodamine-5-maleimide to your research toolkit today.

Related Products

• CyLyte Fluor 3, acid
• CyLyte Fluor 3, NHS ester
• 5(6)-TAMRA [5-(and-6)-Carboxytetramethylrhodamine] "Formulated"

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