SensoLyte® SARS-CoV-2 3CL Protease Activity Assay Kit *Fluorimetric*
$685.00
The SensoLyte® 520 SARS-CoV-2 3CL Protease Activity Assay Kit employs a fluorescence FRET peptide substrate for the detection of enzyme activity. The sequence of this substrate is based on the site-specific cleavage sequence for coronavirus 3CLpro enzymes.
Overview
The SensoLyte® 520 SARS-CoV-2 3CL Protease Activity Assay Kit is an essential tool for researchers focused on diagnosing and understanding the activity of SARS-CoV-2, the virus responsible for COVID-19. Utilizing a fluorescence FRET peptide substrate, this kit allows for the precise detection of enzyme activity related to the coronavirus 3CLpro enzyme. The substrate’s sequence is designed specifically for the site-specific cleavage by 3CLpro, ensuring accurate results in your experiments.
Key Features
- Designed for detecting SARS-CoV-2 3CL protease activity.
- Utilizes a fluorescence FRET peptide substrate for enhanced sensitivity.
- Monitors HiLyte Fluor™ 488 green fluorescence at excitation/emission = 490 nm/520 nm.
- Optimized for use in various research applications related to COVID-19.
- Compatible with standard laboratory equipment.
Specifications
| Component | Details |
|---|---|
| Detection Method | Fluorescence |
| Substrate Type | FRET Peptide |
| Fluorescence Peak | 490 nm / 520 nm |
| Application | SARS-CoV-2 research |
FAQ
How is the enzyme activity measured using this kit?
The kit measures enzyme activity through the cleavage of the FRET peptide substrate, resulting in an increase in fluorescence that can be detected and quantified.
Can this assay kit be used with other coronaviruses?
While this kit is specifically designed for SARS-CoV-2, researchers may find utility in adapting the assay for other coronaviruses based on the substrate’s sequence.
What equipment is required to run this assay?
A fluorescence plate reader set to the appropriate excitation and emission wavelengths (490 nm and 520 nm) is needed to analyze the results from this kit. For detailed workflow processes, refer to our other assay kits for guidance.
Invest in the SensoLyte® 520 SARS-CoV-2 3CL Protease Activity Assay Kit today to enhance your research capabilities in understanding this critical enzyme’s role in COVID-19. Find it now among our premium assay kits for reliable and effective results.
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