SensoLyte® 520 MMP-12 Assay Kit Fluorimetric – 1 kit
$576.00
SensoLyte® 520 MMP-12 Assay Kit Fluorimetric This substrate showed specificity to MMP-12 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13 and 14.
Overview
Enhance your research with the SensoLyte® 520 MMP-12 Assay Kit Fluorimetric, designed for precise measurement of matrix metalloproteinase (MMP) activity. This kit specifically targets MMP-12, a key enzyme linked to various physiological processes, including those involved in smoke-induced emphysema. By using a 5-FAM fluorophore combined with the QXL520™ quencher, the kit enables continuous monitoring of MMP-12 activity with minimal cross-reactivity to other MMPs.
Key Features
- High specificity for MMP-12
- Minimal cross-reaction with other MMPs, including MMP-1, 2, 7, 8, 9, 13, and 14
- Utilizes a fluorogenic FRET peptide substrate for real-time measurement
- Compatible with various assay formats
- Easy to use, requiring minimal setup
Specifications
| Product Name | SensoLyte® 520 MMP-12 Assay Kit Fluorimetric |
|---|---|
| Usage | Fluorimetric measurement of MMP-12 activity |
| Components | 5-FAM labeled substrate, QXL520™ quencher |
| Storage Conditions | Store at -20°C |
FAQ
What types of samples can I use with this assay kit?
This assay kit is suitable for various biological samples, including cell lysates and tissue homogenates, where MMP-12 activity needs to be measured.
How does the SensoLyte® 520 MMP-12 Assay Kit work?
The kit employs a FRET-based approach using a fluorogenic substrate that emits fluorescence upon cleavage by MMP-12. The increase in fluorescence is directly proportional to enzyme activity, allowing continuous monitoring.
Can I compare MMP-12 activity in different conditions?
Yes, the kit allows you to compare MMP-12 activity under various experimental conditions, making it ideal for assessing the impact of treatments or biological responses.
If you’re looking to measure MMP-12 activity effectively, the SensoLyte® 520 MMP-12 Assay Kit is available for purchase. Ensure you have this essential tool in your laboratory for reliable results. For more assay kits, check out our Assay Kits category.
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