Overview
If you’re looking to study protein phosphatases with precision, the SensoLyte® FDP Protein Phosphatase Assay Kit is a valuable addition to your research toolkit. This fluorimetric assay kit provides a highly sensitive approach to detecting the activity of a variety of phosphatases, including alkaline phosphatases, protein tyrosine phosphatases, and serine/threonine phosphatases. The assay is based on FDP, a fluorogenic substrate that produces a strong fluorescent signal upon hydrolysis, making it an ideal choice for drug screening and enzyme activity assays.
Key Features
- Highly sensitive detection of most phosphatases
- Fluorogenic substrate with a fluorescence emission maximum at 520 nm
- Optimal performance at pH levels above 7.0
- Easy-to-follow protocol that saves you time
- Can be used for various research applications
Specifications
| Parameter | Details |
|---|---|
| Product Format | 1 kit |
| Fluorescence Excitation | 490 nm |
| Fluorescence Emission | 520 nm |
| pH Optimization | > 7.0 |
FAQ
What types of phosphatases can I detect with this kit?
The SensoLyte® FDP Protein Phosphatase Assay Kit is designed to detect a variety of phosphatases, including alkaline phosphatases, protein tyrosine phosphatases, and serine/threonine phosphatases.
How does the fluorescence intensity change with pH?
The fluorescence produced from the hydrolysis of FDP is enhanced when the pH is above 7.0, providing a clearer signal for your analysis.
Can this kit be used for high-throughput screening?
Yes, the straightforward protocol and rapid results make this assay kit suitable for high-throughput screening applications, ensuring you can efficiently evaluate enzyme activity.
Add the SensoLyte® FDP Protein Phosphatase Assay Kit to your research arsenal today and take your phosphatase studies further. Explore more of our relevant products, like the SensoLyte® Anti-Human MOG ELISA Kit or the SensoLyte® Anti-PLP ELISA Kitto enhance your assays and broaden your research capabilities.
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