5-FAM, SE [5-Carboxyfluorescein, SE]

Overview

5-FAM, SE (5-Carboxyfluorescein, SE) is a widely used green fluorescent reagent that excels in labeling peptides, proteins, and nucleotides. It offers excellent performance for fluorescence applications, making it a preferred choice for researchers looking to achieve reliable results in their studies. With its amine-reactive succinimidyl ester format, 5-FAM provides enhanced stability compared to traditional FITC reagents, which often suffer from hydrolysis issues.

Key Features

• Stable bioconjugates, reducing the risk of degradation
• Higher conjugation yields due to less stringent reaction conditions
• Ideal for a variety of applications, including in situ labeling
• Compatible with various fluorescent detection systems
• Available as a standalone reagent for flexible use

Specifications

FAQ

What is 5-FAM, SE used for?

5-FAM, SE is primarily used for in situ labeling of peptides, proteins, and nucleotides in various research applications. Its stability makes it suitable for long-term studies.

How does 5-FAM, SE compare to FITC?

Unlike FITC, 5-FAM, SE forms more stable carboxamides, leading to better conjugation yields and reduced hydrolysis, making it a more reliable choice for many applications.

Can I use 5-FAM, SE for live cell imaging?

Yes, due to its stability and compatibility with various fluorescent detection systems, 5-FAM, SE is suitable for live cell imaging applications.

When you’re ready to enhance your research with a reliable fluorescence labeling solution, consider adding 5-FAM, SE to your collection. Check out our full range of green fluorescent dyes or explore other options like 6-FAM and 6-FAM, SE for your specific needs.

Related Products

• 5-FAM [5-Carboxyfluorescein]