Overview
Introducing Hoechst 33258, a highly effective cell-permeant DNA binding dye that allows for precise visualization of DNA within live cells. This 20 mM solution in water comes in a convenient 5 ml format, making it an essential addition to your laboratory toolkit for studies involving nucleic acids. Whether you’re examining cell division, exploring gene expression, or conducting fluorescence microscopy, Hoechst 33258 provides reliable and consistent results.
Key Features
- Cell-permeable dye for effective DNA binding
- Ideal for live cell imaging and nuclear staining
- Compatible with various fluorescence imaging systems
- Convenient 5 ml solution for flexible use
- Can be used in combination with other fluorescent dyes
Specifications
| Property | Details |
|---|---|
| Product Name | Hoechst 33258 |
| Concentration | 20 mM |
| Volume | 5 ml |
| Solvent | Water |
FAQ
What applications is Hoechst 33258 used for?
Hoechst 33258 is predominantly used for staining DNA in live cells, making it invaluable for applications such as cell cycle analysis, apoptosis studies, and gene expression research. This dye binds to the minor groove of DNA, allowing for accurate visualization under fluorescence microscopy.
How does Hoechst 33258 compare to DAPI?
Both Hoechst 33258 and DAPI are popular DNA binding dyes, but Hoechst 33258 is known for its ability to penetrate live cell membranes more effectively. DAPI is typically used in fixed cells, while Hoechst is preferred for live cell applications due to its superior cell permeability. For an alternative, consider our DAPI product.
Can Hoechst 33258 be used with other fluorescent dyes?
Yes, Hoechst 33258 can be used in conjunction with other fluorescent dyes, allowing researchers to visualize multiple targets simultaneously. This versatility enhances experimental design, particularly in complex assays. For example, you can explore our Hoechst 33342 for additional options.
With Hoechst 33258, you can shop confidently, knowing you’re getting a reliable tool for DNA analysis. Don’t miss out on purchasing this essential reagent today.
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