Hoechst 33342, 20 mM solution in water – 5 ml

Overview

Introducing Hoechst 33342, a highly effective cell-permeant DNA binding dye available in a convenient 5 ml format. This 20 mM solution in water is ideal for researchers focusing on nucleic acid labeling and detection, especially in live cells. Hoechst 33342 selectively binds to the minor groove of the DNA helix, making it a valuable tool for a variety of applications, including fluorescence microscopy, flow cytometry, and cell cycle analysis.

Key Features

• Cell-permeable dye for effective DNA binding
• Fluorescence excitation at blue wavelengths
• Compatible with live-cell imaging
• Low background fluorescence for enhanced clarity
• Ideal for multiplexing with other fluorescent dyes

Specifications

FAQ

How does Hoechst 33342 stain DNA?

Hoechst 33342 binds specifically to the minor groove of double-stranded DNA. When exposed to UV light, it emits a blue fluorescence, which allows for easy visualization under a fluorescence microscope.

Can Hoechst 33342 be used in live cell imaging?

Yes, Hoechst 33342 is cell-permeable, meaning it can enter live cells without causing significant toxicity. This makes it suitable for real-time imaging of cellular processes.

Is Hoechst 33342 compatible with other fluorescent dyes?

Absolutely! Hoechst 33342 can be used in multiplex staining protocols, allowing researchers to visualize different cellular components simultaneously. For example, it pairs well with other dyes such as DAPI or our Protein A-HiLyte™ Fluor 750 Conjugate for complex assays.

Don’t miss out on enhancing your research with Hoechst 33342. Explore our range of labeling and detection products to find complementary reagents for your studies.

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