DAPI [4”,6-Diamidino-2-phenylindole, dihydrochloride]

Overview

DAPI (4′,6-Diamidino-2-phenylindole, dihydrochloride) is a potent fluorescent stain specifically developed for labeling DNA in cells. Known for its ability to bind to the AT-rich regions of DNA, DAPI exhibits remarkable fluorescence enhancement when in the presence of nucleic acids, making it an ideal choice for various biological applications. Whether you’re conducting research in genetics, molecular biology, or cellular studies, DAPI is a reliable option that enhances the visualization of nuclear material under UV light.

Key Features

• Specific binding to DNA, particularly AT-rich sequences
• Strong fluorescence emission in the blue range (<500 nm)
• Excellent for cell cycle studies and nuclear staining
• Compatible with a variety of fixation and permeabilization protocols
• Available in multiple formats to suit your experimental needs

Specifications

FAQ

What applications is DAPI used for?

DAPI is commonly used for fluorescent microscopy to stain DNA in fixed cells. It’s particularly effective in identifying cell nuclei, cell cycle analysis, and studying cellular morphology.

How do I prepare DAPI for use?

DAPI can be prepared by dissolving the powder in a suitable buffer or distilled water, typically achieving a working concentration of 1-10 µg/mL depending on your specific application.

Can DAPI be used with other fluorescent dyes?

Yes, DAPI can be used in conjunction with other fluorescent dyes, such as Hoechst 33342 or Hoechst 33258, to provide multiplex staining in experiments. Be sure to choose dyes with non-overlapping emission spectra for optimal results.

Order your DAPI today and enhance your laboratory’s capabilities. For additional options, explore our collection of other fluorescent labeling dyes and proteins available in our catalog.

Related Products

• Hoechst 33342, 20 mM solution in water – 5 ml
• Hoechst 33258, 20 mM solution in water – 5 ml
• CyLyte Fluor 5, acid

Browse more in Blue (<500 nm).