Overview
The Caspase 1 (ICE) Substrate 3f, fluorogenic is a highly effective tool for researchers focused on the detection of caspase activities. Derived from AFC (7-amino-4-trifluoromethylcoumarin), this substrate is specifically designed for fluorimetric assays, allowing you to monitor caspase activity with precision and accuracy. When cleaved by caspases, the substrate emits a strong fluorescence signal that can be easily detected at wavelengths of 500-510 nm, with excitation occurring at 370-390 nm. This property makes the substrate ideal for various applications in proteomics and cellular studies.
Key Features
- Designed for detection of caspase 1 activity
- Fluorogenic AFC-derived substrate for effective monitoring
- Strong fluorescence upon peptide cleavage
- Compatible with standard fluorimetric detection equipment
- 5 mg quantity for multiple experimental setups
Specifications
| Property | Details |
|---|---|
| Product Name | Caspase 1 (ICE) Substrate 3f |
| Type | Fluorogenic Peptide Substrate |
| Weight | 5 mg |
| Excitation Wavelength | 370-390 nm |
| Emission Wavelength | 500-510 nm |
FAQ
What applications can I use Caspase 1 Substrate 3f for?
This substrate is suitable for a variety of applications including monitoring caspase activity in apoptosis studies, drug development, and cellular signaling research.
How do I use the substrate in my experiments?
To utilize the Caspase 1 (ICE) Substrate 3f, dilute the substrate in an appropriate buffer and incubate it with your sample that contains active caspases. Measure the fluorescence to determine the enzyme activity.
Is this substrate compatible with other assays?
Yes, the fluorogenic properties of the substrate make it compatible with various fluorimetric detection systems, ensuring versatility in your experimental design. You may also want to explore other peptides in our catalog, such as PKG Substrate and Autocamtide-2.
For accurate and reliable results in your research, consider adding Caspase 1 (ICE) Substrate 3f to your lab inventory. This product is now for sale, so shop today to enhance your research capabilities!
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