Bacterial Sortase Substrate III, Abz/DNP – 1 mg

Overview

The Bacterial Sortase Substrate III, Abz/DNP, is a specialized peptide that plays a crucial role in studying Staphylococcus aureus. This substrate is specifically designed to interact with Sortase A (SrtA), a transpeptidase that anchors important virulence and surface proteins to the bacterial cell wall. By recognizing the C-terminal LPXTG motif, SrtA facilitates the formation of vital amide bonds that contribute to the bacterium’s ability to colonize and infect.

Key Features

• Designed to react with Sortase A in Staphylococcus aureus.
• Includes the native substrate Abz-LPETG-Dap(DNP)-NH2, which SrtA cleaves.
• Enables monitoring of substrate cleavage at Ex/Em=320 nm/420 nm.
• Catalyzes the formation of an amide bond between threonine and cell-wall crossbridges.
• Essential for studying bacterial infections and virulence mechanisms.

Specifications

FAQ

What is the role of Sortase A in bacteria?

Sortase A is an enzyme that anchors surface proteins to the bacterial cell wall, playing a vital role in bacterial virulence and colonization.

How can I monitor the activity of Bacterial Sortase Substrate III?

You can monitor the reaction using fluorescence at excitation and emission wavelengths of 320 nm and 420 nm, respectively.

Where can I find related products for peptide research?

Explore our range of peptides and other substrates, including our PKG Substrate and Autocamtide-2 for advanced research needs.

This Bacterial Sortase Substrate III is ideal for researchers looking to understand bacterial mechanisms and develop assays targeting SrtA activity. Shop now and enhance your research with this reliable peptide.

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