Overview
5-FITC (Fluorescein-5-isothiocyanate) is the go-to fluorescent labeling reagent for researchers looking to label proteins, peptides, oligonucleotides, and small organic ligands. Known for its bright green fluorescence, 5-FITC plays a crucial role in various applications, including immunofluorescence and flow cytometry. With a peak absorption and emission spectral range of 500-550 nm, this fluorescent dye provides a strong signal for detection in your assays.
Key Features
- Bright green fluorescence for easy detection.
- Compatible with a wide range of proteins and peptides.
- Ideal for various applications including assays, imaging, and flow cytometry.
- Cost-effective option compared to alternative fluorescent reagents.
- Similar absorption and fluorescence properties to 6-FITC.
Specifications
| Property | Details |
|---|---|
| Catalog Number | 5-FITC |
| Form | Powder |
| Size | 1 g |
| Color | Green |
| Absorption/Emission | 500-550 nm |
FAQ
What applications can I use 5-FITC for?
5-FITC is versatile and widely used in applications such as immunofluorescence staining, flow cytometry, and labeling proteins for various assays.
How does 5-FITC compare to other fluorescent dyes?
While 5-FITC is cost-effective and highly popular, alternative amine-reactive fluorescein derivatives may offer improved stability or different spectral properties. However, for many applications, 5-FITC provides excellent performance.
Can I use 5-FITC for labeling peptides?
Yes, 5-FITC is suitable for labeling peptides, enabling efficient detection and quantification in various biochemical assays.
Explore our full range of fluorescent labeling dyes, including related products like 6-FAM and 5-FAMto find the perfect fit for your research needs.
Enhance your experimental results today by purchasing 5-FITC. With its proven reliability and high performance, this labeling reagent will serve as an essential component in your research toolkit.
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