Protein A, recombinant

Overview

AnaSpec’s recombinant Protein A is a highly effective, non-glycosylated cell wall protein derived from Staphylococcus aureus. This protein exhibits a strong affinity for the Fc region of immunoglobulin molecules from various species, making it an invaluable tool for immunoprecipitation, affinity chromatography, and other binding applications. By utilizing recombinant technology, we ensure that our Protein A consists solely of the five IgG binding domains (labeled A through E), each approximately 60 amino acids in length, without the presence of cysteines. The expression of this protein in E. coli guarantees high purity and consistency, addressing your research needs with reliability.

Key Features

• High affinity binding to the Fc region of immunoglobulins
• Consists solely of IgG binding domains A, B, C, D, and E
• Non-glycosylated for straightforward use in various assays
• Produced in E. coli for high purity and yield
• Ideal for applications such as affinity chromatography and immunoprecipitation

Specifications

FAQ

What applications can I use Protein A for?

Protein A is most commonly used in immunoprecipitation and affinity chromatography to isolate antibodies and their complexes. It can also be utilized in various assays for detecting and quantifying proteins.

How is Protein A different from other forms of IgG binding proteins?

Unlike glycosylated versions, our recombinant Protein A is non-glycosylated, which allows for more straightforward binding and minimizes variability during experiments. The absence of cysteines also ensures a consistent performance across different applications.

Can I use Protein A with different species of immunoglobulins?

Yes, Protein A binds with high affinity to the Fc regions of immunoglobulins from various species, making it suitable for a wide range of research applications.

When you’re ready to enhance your assays, consider pairing Protein A with our Protein A-Biotin Conjugate for added versatility. If you’re looking for non-fluorescent labeling options, check out our full selection in the non-fluorescent category. Additionally, explore other labeling compounds like NDA UltraPure Grade to diversify your experimental toolkit.

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