Overview
The SensoLyte® 520 Calpain Activity Assay Kit provides a reliable method for detecting calpain activity using fluorimetric techniques. This kit is essential for researchers studying intracellular Ca2+ dependent cysteine proteases, particularly calpain isoforms 1 (µ) and 2 (m). These enzymes play critical roles in various cellular processes and diseases, including ischemic injury, muscular dystrophy, diabetes, and Alzheimer’s disease. By utilizing our novel internally quenched 5-FAM/QXL™ 520 FRET substrate, you can accurately measure calpain activity to further your research.
Key Features
- Fluorimetric detection for precise measurements
- Utilizes a novel, internally quenched FRET substrate
- Ideal for studying calpain-related cellular processes
- Suitable for a wide range of biological samples
- Comprehensive protocol included for ease of use
Specifications
| Product Name | SensoLyte® 520 Calpain Activity Assay Kit |
| Format | Fluorimetric |
| Includes | 5-FAM/QXL™ 520 FRET substrate and assay components |
| Storage | -20°C |
| Species Reactivity | Human, mouse, rat |
FAQ
What types of samples can I use with the SensoLyte® 520 Calpain Activity Assay Kit?
You can use a variety of biological samples such as cell lysates, tissue extracts, and serum. It’s essential to ensure that the samples are prepared correctly for the most accurate results.
How do I measure calpain activity using this kit?
Follow the comprehensive protocol provided with the kit. It details each step necessary for preparing samples, setting up the reaction, and measuring fluorescence to determine calpain activity.
Can I use this assay kit for my studies on Alzheimer’s disease?
Yes, the SensoLyte® 520 Calpain Activity Assay Kit is particularly useful for research related to Alzheimer’s and other diseases involving calpain activity. This assay can help you understand the role of calpains in pathological processes.
Order your SensoLyte® 520 Calpain Activity Assay Kit today and discover how this powerful tool can enhance your research. If you’re also interested in quantifying specific antibodies, check out the SensoLyte® Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit or the SensoLyte® Anti-PLP (178-191) IgG Quantitative ELISA Kit to complement your assay needs. Experience reliable results and take your research further with AnaSpec’s quality products.
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