SensoLyte® HABA Biotin Quantitation Kit Colorimetric – 1 kit
$237.00
SensoLyte® HABA Biotin Quantitation Kit Colorimetric for estimating molar ratio of biotin to protein in biotin-protein conjugates or biotin concentration
Overview
The SensoLyte® HABA Biotin Quantitation Kit is designed for scientists who need an accurate and efficient method to estimate the molar ratio of biotin to protein in biotin-protein conjugates or to quantify biotin concentration in solutions. This kit utilizes HABA (4’-hydroxyazobenzene-2-carboxylic acid), a special reagent that exhibits a significant change in absorption when bound to avidin. When biotin competes with HABA for binding sites on avidin, it displaces HABA, which results in a measurable decrease in absorption at 500 nm. This simple yet effective assay is an essential tool for your biotin-related research.
Key Features
- Quick and reliable quantification of biotin concentration
- Colorimetric detection method utilizing HABA and avidin interaction
- Ideal for biotin-protein conjugates
- Easy-to-follow protocol for effective results
- Suitable for a variety of research applications
Specifications
| Product Name | SensoLyte® HABA Biotin Quantitation Kit |
| Application | Biotin quantitation in solutions |
| Assay Type | Colorimetric |
| Detection Wavelength | 500 nm |
| Kit Size | 1 kit |
FAQ
How does the SensoLyte® HABA Biotin Quantitation Kit work?
This kit employs a colorimetric assay where HABA binds to avidin. Through competition, biotin displaces HABA, leading to a drop in absorption at 500 nm, which is measurable and correlates with biotin concentration.
What type of samples can I analyze with this kit?
You can use this kit for various biotin-protein conjugates as well as any solution containing biotin, making it a versatile addition to your research toolkit.
Is there any specific equipment required for this assay?
A standard UV-visible spectrophotometer capable of measuring absorbance at 500 nm is necessary for performing the assay.
Take your biotin quantitation to the next level with the SensoLyte® HABA Biotin Quantitation Kit. Perfect for biochemical and biophysical research, this kit is just one of many products we offer in our assay kits range. For additional applications, consider exploring other options like the SensoLyte® Anti-Human MOG ELISA Kit or the SensoLyte® Anti-PLP ELISA Kit. Invest in your research today and streamline your biotin analysis process!
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