Gelatin, FITC Conjugated – 5 mg

Overview

The Gelatin, FITC Conjugated – 5 mg product is a highly specialized fluorogenic substrate designed for researchers focused on proteolytic activity. This gelatin is heavily labeled with fluorescein isothiocyanate (FITC), providing a vivid fluorescent signal upon digestion. The conjugate acts as an effective substrate for gelatinases and collagenases, enabling you to assess protease activity in your samples with precision. When these enzymes target the gelatin, they cleave it, releasing brightly fluorescent peptides that you can easily monitor. The fluorescence increase is directly proportional to the proteolytic activity, allowing for accurate quantification and comparison among samples.

Key Features

• Heavily labeled with FITC for enhanced fluorescence
• Effective substrate for gelatinases and collagenases
• Fluorescence increase is proportional to proteolytic activity
• Ideal for monitoring protease activity
• Long incubation times can improve sensitivity

Specifications

FAQ

How does the FITC conjugation work?

The FITC conjugation involves attaching a fluorescein molecule to the gelatin, allowing the substrate to emit a fluorescent signal when digested by proteases. This approach provides a clear visual indication of enzyme activity.

Can I use this product with other proteases?

Yes, while the Gelatin, FITC Conjugated is optimized for gelatinases and collagenases, it can also be used with other proteases that can digest gelatin, making it versatile for various applications.

What additional products complement this gelatin substrate?

Consider pairing it with our Elastin, FITC Conjugated for expanded applications in your proteolytic studies, or explore our complete range of fluorogenic substrates for more options.

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