Collagen (Type IV), FAM conjugated – 1 mg

Overview

Introducing our Collagen (Type IV), FAM conjugateda highly effective fluorogenic substrate specifically designed for protease activity assays. This innovative substrate is heavily labeled with a fluorescein derivative, which leads to significant quenching of the fluorescence. Upon protease-catalyzed hydrolysis, the quenching effect is relieved, resulting in the release of brightly green fluorescent dye-labeled peptides. You can directly measure the increase in fluorescence intensity as an accurate reflection of protease activity.

Ideal for high-throughput screening (HTS) assays, this product is particularly suited for evaluating Gelatinase A (MMP-2) and provides critical insights in various research and diagnostic applications. Our fluorogenic substrates are unmatched in their ability to deliver reliable and reproducible results across experiments.

Key Features

• Heavily labeled with a fluorescein derivative
• Quenching of fluorescence enables high sensitivity
• Release of fluorescent peptides upon protease activity
• Ideal for HTS assays targeting Gelatinase A (MMP-2)
• Available in a convenient 1 mg size

Specifications

FAQ

What is the mechanism behind the fluorescence quenching?

The fluorescence quenching occurs due to the heavy labeling of collagen with the fluorescein derivative. This labeling inhibits the fluorescence until the protease catalyzes the hydrolysis, releasing the quenching and resulting in a measurable increase in fluorescence.

What types of assays can I perform with this product?

This collagen FAM conjugated substrate is especially effective for assays focused on protease activities, particularly those aimed at screening Gelatinase A (MMP-2). It can also be used in various other research applications related to protease activity.

Is this product suitable for high-throughput screening?

Yes, the Collagen (Type IV), FAM conjugated is designed for high-throughput screening assays, making it a valuable tool for researchers looking to efficiently assess protease activity across multiple samples.

Take your research to the next level with our FDG fluorescein di-ß-D-galactopyranoside and complement your studies with Elastin, FITC Conjugated. Don’t forget to explore our wide range of fluorogenic substrates to enhance your experimental design.

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