FDG [Fluorescein di-ß-D-galactopyranoside] – 5 mg

Overview

FDG (Fluorescein di-β-D-galactopyranoside) is an exceptional fluorogenic substrate designed specifically for the detection of β-galactosidase. This powerful substrate is colorless and nonfluorescent before hydrolysis, providing a reliable way to visualize enzyme activity. When β-galactosidase catalyzes the hydrolysis of FDG, it converts to highly fluorescent fluorescein, producing an increase in fluorescence around 520 nm. This transition makes FDG one of the most sensitive detection tools available for researchers working with this enzyme.

Key Features

• Highly sensitive for detecting β-galactosidase activity.
• Colorless and nonfluorescent, providing clear initial baseline.
• Hydrolysis produces fluorescein, a bright fluorescent product.
• Optimal spectral properties for most fluorescence instruments (Ex/Em = 492/520 nm).
• Ideal for various applications, including research and diagnostics.

Specifications

FAQ

How does FDG work?

FDG works by being hydrolyzed by β-galactosidase, which transforms the nonfluorescent FDG into a highly fluorescent fluorescein, allowing for easy detection through fluorescence spectroscopy.

What applications is FDG suitable for?

FDG is widely used in research and diagnostic applications, particularly in studies involving gene expression, enzymatic activity assays, and other biochemical analyses where β-galactosidase activity is of interest.

Can FDG be used with other fluorescent labeling dyes?

Yes, FDG can be used in conjunction with other fluorescent labeling dyes to create multi-color assays, enhancing the versatility of your experimental designs. For example, consider using Elastin, FITC Conjugated or Gelatin, FITC Conjugated for your studies.

For more information on our selection of fluorogenic substrates, visit our fluorogenic substrates page.

Related Products

• D-Luciferin, free acid UltraPure Grade – 25 mg