Overview
HiLyte™ Fluor 488 hydroxylamine, HCl salt single isomer is an innovative carbonyl-reactive labeling dye designed for researchers focusing on the detection of aldehydes within proteins and DNA. This unique dye reacts preferentially with aldehydes at physiological pH, making it a reliable choice for various biological applications. By forming stable oxime ethers, it ensures accurate detection without compromising the integrity of your samples.
Key Features
- Highly selective carbonyl-reactive labeling dye.
- Effective at physiological pH for superior results.
- Stable reaction products for reliable detection.
- Ideal for labeling proteins and nucleic acids.
- Extinction coefficient of 70,000 M-1cm-1.
Specifications
| Property | Value |
|---|---|
| Product Name | HiLyte™ Fluor 488 hydroxylamine, HCl salt single isomer |
| Weight | 1 mg |
| Fluorescence Emission | Green (500 -550 nm) |
| Reactivity | Aldehydes |
FAQ
How does HiLyte™ Fluor 488 hydroxylamine work?
This dye reacts specifically with aldehydes to form stable oxime ethers, allowing for direct detection in experiments.
Can I use this dye for labeling proteins?
Yes, HiLyte™ Fluor 488 hydroxylamine is perfect for labeling proteins, ensuring clear visualization in your assays.
What are the optimal conditions for using this dye?
The dye works best at physiological pH, which enhances its reactivity with aldehydes.
In your research, using HiLyte™ Fluor 488 hydroxylamine can significantly improve the accuracy of your labeling experiments. For additional related products, explore our selection of fluorescent labeling dyes in the Green (500 -550 nm) category, or consider testing our 5-FAM and 6-FAM, SE products for additional options in your assays. Ensure your research gets the clarity and precision it needs with HiLyte™ Fluor 488 hydroxylamine, available now for purchase.




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