Collagen (Type I), FITC conjugated Water Insoluble – 5 mg

Overview

Introducing our Collagen (Type I), FITC Conjugated Water Insoluble – 5 mga top-quality fluorogenic substrate designed specifically for researchers and professionals in the field of biochemistry. This water-insoluble form of collagen enables precise fluorometric measurements of collagenase activity, allowing you to observe enzymatic processes with clarity. By utilizing this FITC-conjugated collagen, you can enhance your assays and gain deeper insights into the dynamics of collagen degradation.

Key Features

• Designed for fluorometric measurement of collagenase activity.
• Heavily labeled with FITC to ensure optimal fluorescence quenching.
• Release of brightly green fluorescent dye-labeled peptides upon proteolysis.
• Increase in fluorescence intensity directly correlates with protease activity.
• Perfect for both research and diagnostic applications.

Specifications

FAQ

How does the FITC conjugation affect fluorescence intensity?

The FITC conjugation significantly enhances the detection capabilities as it is heavily labeled, allowing for almost total quenching of the conjugates’ fluorescence prior to proteolysis. This means that once the collagenase activity begins, the release of fluorescent peptides will result in a measurable increase in fluorescence intensity, enabling accurate quantification of enzymatic activity.

Can I combine this product with other substrates?

Yes, this water-insoluble collagen can be effectively used alongside other substrates, such as Elastin, FITC Conjugated – 1 mg and Gelatin, FITC Conjugated – 5 mgfor a comprehensive analysis of protease activity. Tailoring your assay strategy with multiple substrates can yield richer data and insights.

What applications is this product suitable for?

This product is ideal for a wide range of applications in biochemical research, including studies on extracellular matrix remodeling, cancer research, and wound healing. Its capability for fluorometric measurement makes it particularly useful in situations where precise enzymatic activity is needed. For additional fluorogenic substrates, consider checking our complete range of fluorogenic substrates.

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